Skip to product information
1 of 5

PLAP/AALP Human Recombinant Protein

PLAP/AALP Human Recombinant Protein

Regular price $115.00
Regular price Sale price $115.00
Sale Sold out
Shipping calculated at checkout.
Size

Product Description

Alkaline phosphatase, placental type is a membrane-bound glycosylated
dimeric enzyme with a transmembrane domain that is removed in the
mature form, also referred to as the heat-stable form, that is expressed
primarily in the placenta, although it is closely related to the intestinal form
of the enzyme as well as to the placental-like form.

Description: Recombinant human PLAP produced in plants has a predicted molecular weight of 60-65kDA. It does not contain tags.

Our products are produced using a proprietary plant based expressed system.

  • Animal Component Free (ACF). No endotoxins, prions or virons to risk microbial contamination.
  • Highly flexible platform used to produce multiple proteins with minimal changes to method.
  • Our proteins generally have 10% to 20% higher activity.

Purchase Through Fisher Scientific (No Discount)

Price Match Guarantee

If you find a lower price of PLAP recombinant protein, send us a copy of the estimate or a link to the online listing and we'll match the price for quantity. It's that EASY! Send to orders@mnpharm.com.

Current Lead Time

1-2 Working Days 

Product Specifications

Species
Human*
Expression System Nicotiana Benthamiana
Purity >95% by SDS-PAGE
Activity
0.3-3.75 ng/mL EC50
Activity Assay Induced HUVEC proliferation assay data
Cell proliferation is measured by Rezasurin
rhVEGF165 MNPHARM, ED50 = 4.557 ng/mL
Using 2 mg/mL concentration measurement
Animal Component Free (ACF) Yes
Molecular Weight
60-65 kDA
Structure Disulfide-linked homodimer
Endotoxin Concentration
<1 EU/µg
Form Frozen
Formulation
PLAP delivered soluble/frozen carrier-free, does not contain BSA. 50mM Tris, 10% glycerol, pH 7.8
Purification Sequential Chromatography
Tags None
Stability Duration 6 Months

*For Research Use Only

 

MNPharm Guarantee

If your product PLAP is within its expiration period, and has not performed according to expectations, fill out the sample return form. Return the unused sample frozen, with the auto generated packing slip to the address on the instructions. You will receive a full refund along with a replacement of the unused portion (inclusive of the failed test). Damages do not cover any additional costs incurred for failed experimentation. 

Storage Instructions

Until use, ALPP Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a
carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Sample Prep Instructions 

  1. Thaw the vial on ice, spin briefly
  2. Prepare single-use or stock aliquots. This stage will depend on your final application so please adapt as appropriate. All our proteins are supplied carrier protein-free. If compatible with your work and you are storing at lower concentrations (<50 ug/ml) adding carrier protein is highly advised (usually 1% w/v high purity BSA or equivalent – ensure all buffers are sterile-filtered).
  3. Prepare single-use aliquots whenever possible to avoid freeze-thaw cycles which can damage the proteins and reduce bioactivity. Store aliquots at -70 °C (or -20 °C)
  4. We recommend sterile filtering after dilution in media or the final working solution

Bioinformatics

Alternative Names: Alkaline phosphatase, placental; ALP; IAP; ALPI; PALP; PLAP; PLAP-1

UniProt ID: (Human) Q9Y263

Entrez Gene ID: (Human) 250

Amino Acid Sequence: MLGPCMLLLL LLLGLRLQLS LGIIPVEEEN PDFWNREAAE 
ALGAAKKLQP AQTAAKNLII FLGDGMGVST VTAARILKGQ KKDKLGPEIP LAMDRFPYVA LSKTYNVDKH VPDSGATATA YLCGVKGNFQ TIGLSAAARF NQCNTTRGNE VISVMNRAKK AGKSVGVVTT TRVQHASPAG TYAHTVNRNW YSDADVPASA RQEGCQDIAT QLISNMDIDV ILGGGRKYMF RMGTPDPEYP DDYSQGGTRL DGKNLVQEWL AKRQGARYVW NRTELMQASL DPSVTHLMGL FEPGDMKYEI HRDSTLDPSL MEMTEAALRL LSRNPRGFFL FVEGGRIDHG HHESRAYRAL TETIMFDDAI ERAGQLTSEE DTLSLVTADH SHVFSFGGYP LRGSSIFGLA PGKARDRKAY TVLLYGNGPG YVLKDGARPD VTESESGSPE YRQQSAVPLDE ETHAGEDVAV FARGPQAHLV HGVQEQTFIAH VMAFAACLEP YTACDLAPPA GTTDAAHPGR SVVPALLPLL AGTLLLLETA TAP

Documents & Downloads

      

COA included in purchase

 

 References


View full details